1,10-PHENANTHROLINE HYDROCHLORIDE MONOHYDRATE



Synonym : 1,10-Phenanthroline chloride monohydrate,
o-Phenanthroline hydrochloride monohydrate.
CAS No. : 3829-86-5
Mol. Formula : C12H8N2 • HCl • H2O
Molecular Wt. : 234.68
Description : White to light yellow to light purple powder
Ign. Residue (as SO4) : Max.0.2%
Loss on drying at 105°C : Max. 8%
Melting Point : 224°C - 227°C
Assay : Min. 97%

Infrared spectrum Conforms to Structure
Carbon 59.3 - 63.5 %
Nitrogen 11.5 - 12.4 %
Titration by AgNO3 96.5 - 103.5 %
Purity (HPLC) ≥96.5 %
Purity (Titration) >99.995%

Application
RNAs from wastewater samples and reference samples were fragmented and labeled by the hydroxyl radical method using the following reactant concentrations:
5mM o-phenanthroline hydrochloride monohydrate, 500 mM CuSO4, 1mM lissamine-rhodamine B ethylenediamine (Molecular Probes, Inc., Eugene, OR), 4mM H2O2, 20mM sodium phosphate, and 20mM NaCNBH3.After fragmentation and labeling the RNA concentration was determined by spectrophotometric analysis at 260 nm.